These studies were based on inactivated spore preparations only, and the broad applicability of these techniques to environmental samples is unknown. Molecular basis for dimethylsulfoxide DMSO action on lipid membranes. Marine Ecol Prog Ser. Advertisement Hide. We describe the compatibility of these viability assessments with high-throughput quantification techniques, including flow cytometry and quantitative PCR qPCR.
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Such extreme sample loss is particularly important to consider for low-biomass studies, which may have insufficient RNA yields [ 20 ]. J Microbiol. Depth distribution of active bacteria and bacterial activity in lake sediment. Nucl Acid Res. Quantification of cell specific uptake activity of microbial products by uncultured Chloroflexi by microautoradiography combined with fluorescence in situ hybridization. Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes.